Effects of perinatal stress on the metabolites and lipids in plasma of dairy goats

Abstract Dairy goats experience metabolic stress during the peripartal period, and their ability to navigate this stage of lactation is related occurrence development diseases. Unlike dairy cows, there a lack comprehensive analysis changes in plasma profiles goats, particularly using high-throughput techniques. A subset 9 clinically-healthy were used from cohort 96 primiparous Guanzhong (BCS, 2.75 ± 0.15). Blood samples collected at seven time points around parturition (d 21, 14, 7 before parturition, day kidding, d 7, 21 postpartum), analyzed untargeted metabolomics targeted lipidomics. The orthogonal partial least squares discriminant model revealed total 31 differential metabolites including p-cresol sulfate, pyruvic acid, cholic oxoglutaric acid. pathway enrichment identified phenylalanine metabolism, aminoacyl-tRNA biosynthesis, citrate cycle as top three significantly-altered pathways. Limma package 123 differentially expressed lipids. Phosphatidylserine (PS), free fatty acids (FFA), acylcarnitines (ACs) significantly increased on while diacylglycerols (DAG) triacylglycerols (TAG) decreased. Ceramides (Cer) lyso-phosphatidylinositols (LPI) postpartum PS, FFA, ACs decreased gradually returned antepartum levels. Individual species FFA phosphatidylcholines (PC) segregated based differences saturation length carbon chain. Overall, work generated largest repository lipidome metabolome across which contributed our understanding multifaceted adaptations transition goats.